Resveratrol inhibits autophagy and promotes apoptosis in uveal melanoma cells via miR-512-3P/DUSP1 axis

Aim To investigate the regulatory role and mechanism of resveratrol in inhibiting autophagy and promoting apoptosis in choroidal melanoma cells. Methods Choroidal melanoma cells (MUM2B) were divided into control and experimental groups, and treated with different concentrations of resveratrol (0, 10, 20, 40, 60, 80 μmol·L^-1). Cell migration was detected by scratch assay, cell viability was detected by CCK-8, cell apoptosis was detected by flow cytometry, and the expressions of miR-512-3P, DUSP1, and other target proteins were detected by qRT-PCR and Western blot. miR-512-3P was verified to be targeted to the silencing of DUSP1 by RIP. The biological significance of miR-512-3P was assessed in vitro using miR-512-3P mimic. Results Resveratrol inhibited the proliferation and invasion of MUM2B cells, attenuated cellular autophagy, and promoted apoptosis. In addition, cellular p-ERK and p-MTOR protein expression significantly increased, and miR-512-3P expression increased. Overexpression of miR-512-3P targeted and inhibited DUSP1 expression and promoted apoptosis in MUM2B cells. Conclusion Resveratrol targets and inhibits DUSP1 by promoting miR-512-3P expression, thereby inhibiting autophagy and promoting apoptosis in uveal melanoma cells.